Chromator is required for proper microtubule spindle formation and mitosis in Drosophila |
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Authors: | Yun Ding,Mariana Lince-Faria,Weili Cai,Jack Girton,Jø rgen Johansen |
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Affiliation: | a Department of Biochemistry, Biophysics, and Molecular Biology, 3156 Molecular Biology Building, Iowa State University, Ames, IA 50011, USA b Instituto de Biologia Molecular e Celular, Faculdade de Medicina, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal c Laboratory for Cell and Molecular Biology, Faculdade de Medicina, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal |
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Abstract: | The chromodomain protein, Chromator, has been shown to have multiple functions that include regulation of chromatin structure as well as coordination of muscle remodeling during metamorphosis depending on the developmental context. In this study we show that mitotic neuroblasts from brain squash preparations from larvae heteroallelic for the two Chromator loss-of-function alleles Chro71 and Chro612 have severe microtubule spindle and chromosome segregation defects that were associated with a reduction in brain size. The microtubule spindles formed were incomplete, unfocused, and/or without clear spindle poles and at anaphase chromosomes were lagging and scattered. Time-lapse analysis of mitosis in S2 cells depleted of Chromator by RNAi treatment suggested that the lagging and scattered chromosome phenotypes were caused by incomplete alignment of chromosomes at the metaphase plate, possibly due to a defective spindle-assembly checkpoint, as well as of frayed and unstable microtubule spindles during anaphase. Expression of full-length Chromator transgenes under endogenous promoter control restored both microtubule spindle morphology as well as brain size strongly indicating that the observed mutant defects were directly attributable to lack of Chromator function. |
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Keywords: | Chromator Spindle-assembly checkpoint Mitosis Spindle matrix Drosophila |
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