Fragment of the gene encoding chymotrypsin and trypsin inhibitor protein of potato tubers |
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| Authors: | I A Parfenov T A Revina P P Pashkovsky N L Radyukina T A Valueva |
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| Institution: | 1.Bach Institute of Biochemistry,Russian Academy of Sciences,Moscow,Russia;2.Timiryazev Institute of Plant Physiology,Russian Academy of Sciences,Moscow,Russia |
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| Abstract: | Product of polymerase chain reaction designated as PKPIJ-B was isolated after amplification from genomic DNA of potato (Solarium tuberosum L., Zhukov Jubilee cultivar) using the designed primers. Nucleotide sequence of PKPIJ-B was determined and amino acid sequence of protein was restored. Analysis of this sequence has allowed us to suggest that
isolated gene fragment encodes chymotrypsin and trypsin inhibitor protein (PKCI-23 potato Kunitz-type chymotrypsin inhibitor)
of potato tubers. |
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