Purification and characterization of cytoplasmic 14C-arginine rich basic proteins of Ehrlich ascites tumor cells |
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Authors: | Ryszard Farbiszewski Danuta Kilczewska |
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Institution: | (1) Department of Inorganic and Analytic Chemistry, Institute of Chemistry and Biophysics, Medical School, Bialystok 8, Poland |
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Abstract: | Summary Arginine rich basic proteins from cytoplasm of Ehrlich ascites tumor cells have been separated and partially characterized. All proteins show a cationic character with the following isoelectric points: 8.45, 8.60, 8.70, 8.90, and possess various amount of arginine. The protein of the highest molecular weight (75000) has the greatest amount of arginine (18.1%), specific radioactivity (19760 cpm/mg, min) and isoelectric point (8.9). The significance of those proteins in the cytoplasm of tumor cells has been discussed briefly. |
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