Formation and analysis of heterocyclic aromatic amine-DNA adducts in vitro and in vivo |
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Authors: | Turesky Robert J Vouros Paul |
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Institution: | Division of Chemistry, National Center for Toxicological Research, 3900 NCTR Road, Jefferson, AR 72079, USA. rturesky@nctr.fda.gov |
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Abstract: | The detection and quantification of heterocyclic aromatic amine (HAA)-DNA adducts, critical biomarkers in interspecies extrapolation of toxicity data for human risk assessment, remains a challenging analytical problem. The two main analytical methods currently in use to screen for HAA-DNA adducts are the 32P-postlabeling assay and mass spectrometry, using either accelerated mass spectrometry (AMS) or liquid chromatography and electrospray ionization mass spectrometry (LC-ESI-MS). In this review, the principal methods to synthesize and characterize DNA adducts, and the methods applied to measure HAA-DNA adduct in vitro and vivo are discussed. |
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Keywords: | Reviews Heterocyclic aromatic amine–DNA adducts DNA |
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