Characterization of a novel lipid A structure isolated from Azospirillum lipoferum lipopolysaccharide |
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Authors: | Choma Adam Komaniecka Iwona |
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Institution: | Department of General Microbiology, Maria Curie-Sklodowska University, 19, Akademicka St., 20-033 Lublin, Poland. achoma@hektor.umcs.lublin.pl |
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Abstract: | The structure of lipid A from Azospirillum lipoferum, a plant-growth-promoting rhizobacterium, was investigated. It was determined by chemical analysis, mass spectrometric methods, as well as 1D and 2D NMR spectroscopy. Because of the presence of substituents, the investigated lipid A differs from typical enterobacterial lipid A molecules. Its backbone is composed of a beta-(1,6)-linked D-glucosamine disaccharide but lacks phosphate residues. Moreover, the reducing end of the backbone (position C-1) is substituted with alpha-linked d-galacturonic acid. 3-hydroxypalmitoyl residues are exclusively connected to amino groups of the glucosamine disaccharide. Hydroxyls at positions C-3 and C-3' are esterified with 3-hydroxymyristic acids. Primary polar fatty acids are partially substituted by nonpolar fatty acids (namely, 18:0, 18:1 or 16:0), forming acyloxyacyl moieties. |
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Keywords: | Azospirillum lipoferum Lipid A structure Glucosamine |
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