RNA Polymerase B levels during the cell cycle ofPhysarum polycephalum |
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Authors: | Gérard Pierron Helmut W Sauer |
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Institution: | (1) Zoologisches Institut der Universität, Röntgenring 10, D-8700 Würzburg, Federal Republic of Germany |
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Abstract: | Summary Tritiated -amanitin has been used as a specific and sensitive probe to estimate the number of RNA polymerase B molecules in isolated nuclei, chromatin and nucleoids, obtained from macroplasmodia ofPhysarum polycephalum. During mitosis (metaphase±10 min) there is at least 10-fold less RNA polymerase B than at all phases of the cell cycle, even if DNA replication has been blocked in vivo. It is concluded that many of the RNA polymerase B molecules leave the chromatin during decondensation of the chromosomes in telophase of the synchronous nuclear division ofPhysarum. |
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Keywords: | RNA polymerase B [3H]-amanitin binding" target="_blank">gif" alt="agr" align="BASELINE" BORDER="0">-amanitin binding Cell cycle Physarum |
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