Unusual base sequence arrangement in phage phi 29 DNA. |
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Authors: | J Ito R J Roberts |
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Institution: | 1. Department of Cellular Biology, Scripps Clinic and Research Foundation, La Jolla, CA 92037 USA;2. Cold Spring Harbor Laboratories, Cold Spring Harbor, NY 11724 U.S.A. |
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Abstract: | Susceptibility of Bacillus subtilis phage phi 29 DNA to 34 different restriction endoculceases was determined. Three enzymes, BglI, XbaI and BstEII, were found to cleave phi 29 DNA only once at specific sites. The sites of these single cleavages have been mapped. Thirteen enzymes did not cut phi 29 DNA. phi 29 HindIII DNA fragments inserted into pBR313 plasmid and propagated in Escherichia coli, were resistant to these restriction endonucleases. This result suggests that the insusceptibility is due to the absence of the nucleotide sequences on phi 29 recognized by the enzymes, and not to the presence of modified nucleotides. |
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Keywords: | Endonucleolytic cleavage recombinant DNA plasmid-φ29 hybrids |
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