Germination of Pistacia vera L. pollen in liquid medium |
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Authors: | A Golan-Goldhirsh U Schmidhalter M Müller J J Oertli |
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Institution: | (1) The Jacob Blaustein Institute for Desert Research, Sede Boqer Campus, Ben-Gurion University of the Negev, 84993, Israel;(2) ETH, Institut für Pflanzenwissenschaften, Versuchsstation Eschikon 33, CH-8315 Lindau (ZH), Switzerland;(3) ETH, Labor für EM I, Institut für Zellbiologie, CH-8092 Zürich, Switzerland |
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Abstract: | Summary The osmotic effect of Polyethylene glycol (PEG) has been shown to be sufficient to induce the germination of Pistacia vera L. pollen in liquid medium. The prehydration of the pollen in a saturated atmosphere for approximately 10 h was necessary to obtain maximum in vitro germination. Imbibition of the pollen in water resulted in the rapid leakage of solutes into the medium. These solutes consisted of approximately 50% carbohydrates, of which sucrose (0.65 mol/mg), glucose (0.77 mol/mg) and fructose (0.78 mol/mg) were the major sugars; the remaining 50% comprised proteins with the following major molecular weights 63 kDa, 60 kDa, 59 kDa, 40 kDa, 36 kDa, 35.5 kDa, 31 kDa, other organic matter and minerals. |
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Keywords: | Pistacia vera L In vitro germination Pollen Osmotic effect |
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