A convenient assay for ADP reductase activity using Dowex-1-borate columns |
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Authors: | J G Cory F A Russell M M Mansell |
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Institution: | 1. Department of Medical Microbiology, University of South Florida, Tampa, Florida 33620 USA;2. Department of Chemistry, University of South Florida, Tampa, Florida 33620 USA |
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Abstract: | A convenient assay for ADP reductase activity has been developed in which 14C]deoxyadenosine is separated from 14C]adenosine on Dowex-1-borate columns. The separation is complete and the recovery of deoxyadenosine is quantitative. Using disposable Pasteur pipets as the columns, a large number of ADP reductase assays can be handled at one time. |
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