Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum |
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Authors: | Gopalakrishnan Subramanium Beale Michael H Ward Jane L Strange Richard N |
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Affiliation: | Department of Biology, Darwin Building, University College London, Gower Street, London WC1E 6BT, UK. |
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Abstract: | Fusarium acutatum was isolated from wilting chickpea plants in Pakistan. Filtrates from cultures grown on a defined liquid medium caused permanent wilting of chickpea cuttings and killed cells, isolated enzymically from healthy plants, in a bioassay. Toxic activity was retained by a cyano solid phase extraction cartridge and the toxin was isolated by elution from the cartridge in acetonitrile and Si-gel thin layer chromatography of the eluate. Analytical HPLC of the compound on a cyano column with diode array detection gave a single peak with a homogeneous spectrum and lambda(max) at 224 and 281 nm. NMR and mass spectral studies showed that the toxin was 8-O-methyl-fusarubin. The pure compound caused permanent wilting of chickpea cuttings and the LD50 value in the cell bioassay was 327 ng/ml. |
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Keywords: | Cicer arietinum Leguminosae Chickpea Fusarium acutatum Wilt Bioassay NMR Mass spectrum Naphthoquinone Phytotoxin |
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