A Class II fructose-1,6-bisphosphate aldolase from a halophilic archaebacterium Haloferax mediterranei |
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| Authors: | D'Souza Sandra E Altekar Wijaya |
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| Institution: | Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India. |
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| Abstract: | Fructose-1,6-bisphosphate (FBP) aldolase (EC 4.1.2.13) was purified 97-fold from a halophilic archaebacterium Haloferax mediterranei, with a specific activity of 2.8. The enzyme was characterized as a Class II aldolase on the basis of its inhibition by EDTA and other metal chelators. The enzyme had a specific requirement for divalent metal Fe(2+) for activity. Sulfhydryl compounds enhanced aldolase activity. |
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