Partial characterization of a 36-kDa protein from Clostridium botulinum type E that inhibits trypsin and chymotrypsin |
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| Authors: | Prabakaran Sakthivel DasGupta Bibhuti R |
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| Institution: | Department of Food Microbiology and Toxicology, University of Wisconsin, 1925 Willow Drive, Madison, WI 53706, USA. |
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| Abstract: | A 36-kDa trypsin inhibitor was purified from Clostridium botulinum type E culture supernatant by multiple molecular sieve and ion exchange chromatographic steps. The sequence of the amino-terminal 13 amino acid residues of this single-chain protein is Asn.Gln.Glu.Val.Phe.Asn.Met.Pro.Lys.Phe.Ser.Thr.Ala-. This novel protein that also inhibits chymotrypsin is produced by an organism that does not appear to produce any protease. |
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