Impact of copy number of distinct SV40PolyA segments on expression of a GFP reporter gene |
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| Authors: | Kang Yin XiuFang Wang Huan Ma Ying Xie JingJing Feng QinQing Yang ZhanJun Lü |
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| Institution: | 1.Hebei Key Lab of Laboratory Animal, Department of Genetics,Hebei Medical University,Shijiazhuang,China |
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| Abstract: | The presence of Alu repeats downregulates the expression of the green fluorescent protein(GFP) gene.We found that SV40PolyA(PolyA,240 bp),in either orientation,eliminated the inhibition of GFP gene expression induced by Alu repeats when it was placed between the GFP gene and the Alu repeats.In this study,4 different segments(each 60 bp) were amplified from antisense PolyA(PolyAas) by PCR,and inserted upstream of Alu14 in pAlu14 plasmid(14 Alu repeats inserted downstream of the GFP gene in vector pEGFP-C1 in... |
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| Keywords: | copy number SV40PolyA repeat sequence GFP Alu |
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