An efficient fluorimetric method to measure the viability of intraerythrocytic Plasmodium falciparum |
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Authors: | Evers Astrid Heppner Saskia Leippe Matthias Gelhaus Christoph |
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Affiliation: | 1Department of Zoophysiology, Zoological Institute, University of Kiel, Olshausenstr. 40, D-24098 Kiel, Germany. |
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Abstract: | A range of various assays to measure chemosusceptibility of Plasmodium falciparum have been described in the literature. As the screening of a plethora of compounds for antiplasmodial activity is urgently needed and becomes a constantly increasing routine analysis, a test system has to fulfill the following requirements: sensitivity, reliability, simplicity of performance, high-throughput compatibility, and cost-effectiveness. Here, we describe an assay that fulfills all criteria and in which the fluorescent SYTOX Green dye is introduced to determine growth inhibition of Plasmodia in in vitro cultures. |
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