Induction of cytosolic aspartate aminotransferase by glucagon in primary cultured rat hepatocytes |
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| Authors: | Y Horio H Fukui M Taketoshi T Tanaka H Wada |
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| Institution: | Department of Pharmacology II, Osaka University School of Medicine, Japan. |
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| Abstract: | The activity and the mRNA content of cytosolic aspartate aminotransferase (EC 2.6.1.1) were examined in cultured rat hepatocytes. Addition of glucagon (1 x 10(-7) M) in the presence of dexamethasone (1 x 10(-7) M) caused about 2-fold increase in the activity and mRNA content. Dibutyryl cAMP (1 x 10(-4) M) could replace glucagon for this effect. Maximal induction of cytosolic aspartate aminotransferase mRNA was observed 8 h after their additions. Insulin (1 x 10(-7) M) did not inhibit the enzyme induction by glucagon or dibutyryl cAMP. These results suggest that the cytosolic aspartate aminotransferase gene is regulated by cAMP, and not by insulin. |
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