Molecular cloning,sequence and expression analysis of <Emphasis Type="Italic">ZmArf2</Emphasis>, a maize ADP-ribosylation factor |
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Authors: | Yanyang Liu Junzhou Li Yuling Li Mengguan Wei Qingxin Cui Qilei Wang |
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Institution: | (1) College of Agriculture, Henan Agricultural University, 95 Wenhua Rd, Zhengzhou, China; |
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Abstract: | A full-length cDNA encoding a maize GTP-binding protein of the ADP-ribosylation factor family was cloned by suppression subtractive
hybridization and an in silico cloning approach. The cDNA was 938 bp in length and contained a complete ORF of 612 bp, which
encodes a protein of 203 amino acid residues. Its deduced amino acids sequence had an 83% identity with that of a GTP-binding
protein in rice. The gene was designated ZmArf2. The ZmArf2 gene consists of G1, G2, G3, G4 and G5 boxes, and Switch I and Switch II regions. Eight nucleotides differed and five amino
acids changed between the popcorn inbred N04 and the dent corn inbred Dan232. One changed amino acid was in the G1 box. RT-PCR
analysis showed that ZmArf2 expression increased in the early stages of endosperm development and was not tissue-specific. |
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