Increased synthesis of human parathyroid hormone in Escherichia coli through alterations of the 5' untranslated region |
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| Authors: | G Morelle H Mayer |
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| Institution: | GBF-Gesellschaft für Biotechnologische Forschung mbH, Abteilung Genetik, Braunschweig, F.R.G. |
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| Abstract: | The expression of human parathyroid hormone (hPTH) in Escherichia coli was optimized by variations of the spacing sequence between the ribosome-binding site (RBS) and the beginning of the gene (ATG) and by increasing the complementarity of the RBS to the 16 S rRNA. The expression level of 3 micrograms/liter increased more than 100-fold to 475 micrograms/liter as a direct consequence of modifications in the region 5' of the gene. |
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