Solubilization of adrenal medullary opioid receptors |
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Authors: | K Kamikubo H Murase M Murayama K Miura |
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Institution: | Third Department of Internal Medicine, Gifu University School of Medicine, Japan. |
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Abstract: | The opioid-binding activity of digitonin extract of bovine adrenal medullary membranes was studied. 3H]Diprenorphine binding to the solubilized material was rapid and saturable. The dissociation constant of 3H]diprenorphine binding was 0.76 nM. Several opioids displaced the 3H]diprenorphine binding. The complex of 3H]diprenorphine and the solubilized binding sites was eluted as a single peak on a Sepharose 6B column and showed an apparent molecular weight of 200,000. These results indicate that active opioid receptors are solubilized with digitonin from bovine adrenal medullary membranes. |
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