Some peculiarities of the reaction kinetics of ATP hydrolysis by Ca2+-dependent ATPase of chloroplasts]

The dependence of the reaction rate of ATP hydrolysis by CF1-ATPase of chloroplasts on the substrate (CaATP) concentration is of complex nature. It is assumed that such dependence may be due to either conformational changes of the enzyme during the increase in CaATP content in the reaction medium, or consecutive binding of the substrate to its active sites, differing in their affinity to the substrate of the reaction.