Photolysis of caged Ca2+ induces trichocyst discharge inParamecium caudatum |
| |
Authors: | Y Iwadate M Kikuyama H Asai |
| |
Institution: | (1) Department of Physics, Waseda University, Shinjuku-ku, Tokyo;(2) Biological Laboratory, The University of the Air, Wakaba, Mihama-ku, 261-8586 Chiba, Japan |
| |
Abstract: | Summary Trichocyst discharge, ciliary reversal, and cell body contraction inParamecium spp. have all been claimed to be regulated by the intracellular Ca2+ concentration (Ca2+]i) at the cortical region of the cell. We injected caged Ca2+ intoP. caudatum cells and applied ultraviolet (UV) light to the cell for 125 ms. This did not induce trichocyst discharge but did induce both ciliary reversal and cell body contraction. A re-application of UV for 125 ms triggered trichocyst discharge. These results demonstrate that (1) trichocyst discharge and ciliary reversal and cell body contraction are controlled by Ca2+]i and (2) the threshold of Ca2+]i for trichocyst discharge is higher than those for the other two functions.Abbreviations DTT
dithiothreitol
- EGTA
ethylene glycol-bis( -aminoethyl ether)-N,N,N ,N -tetraacetic acid
- ICL
infraciliary lattice
- Ca2+]i
intracellular Ca2+ concentration
- NP-EG
o-nitrophenyl EGTA
- PMT
photomultiplier tube
- UV
ultraviolet |
| |
Keywords: | Caged calcium Ciliary reversal Ciliate Nitrophenyl EGTA |
本文献已被 SpringerLink 等数据库收录! |
|