感染球孢白僵菌后红火蚁体内蛋白质含量的变化

为了解球孢白僵菌Beauveria bassiana成功感染红火蚁Solenopsis invicta的生理生化机制, 本研究在室内条件下测定了经球孢白僵菌感染后红火蚁工蚁、蛹和幼蚁体内蛋白质含量的变化。结果表明: 红火蚁各虫态感染球孢白僵菌后体内蛋白质含量变化存在差异。在接种后12-72 h, 工蚁体内蛋白质含量在24 h时最高, 为47.12 mg/g, 显著低于对照的56.40 mg/g(P＜0.05), 此后呈下降趋势, 72 h时蛋白质含量下降至25.16 mg/g。幼蚁在接种36 h时体内蛋白质含量最高, 为24.13 mg/g, 此后呈下降趋势, 72 h时蛋白质含量为8.95 mg/g, 显著低于对照的24.80 mg/g。蛹感染球孢白僵菌后体内蛋白质含量一直呈下降趋势, 从12 h的36.57 mg/g降到72 h的10.42 mg/g, 与对照的38.61 mg/g相比差异显著(P＜0.05)。结果显示球孢白僵菌感染能显著降低红火蚁各虫态体内蛋白质含量。

Changes of protein content in the red imported fire ant(Solenopsis invicta)infected by Beauveria bassiana

In order to understand the physiological and biochemical mechanism that Beauveria bassiana successfully infected the red imported fire ant (RIFA), Solenopsis invicta, the protein content in infected adults, larvae and pupae of RIFA was determined in the laboratory. Following fungal infections, the protein content varied in different developmental stages of infected RIFA. At 12-72 h after inoculation, the protein content in worker body reached as high as 47.12 mg/g at 24 h, showing significant difference from control group (56.40 mg/g)(P＜0.05), and then dropped to 25.16 mg/g at 72 h. The highest protein content of 24.13 mg/g was observed in larval body at 36 h after inoculation. After that, the protein content in larval body decreased, and was 8.95 mg/g at 72 h after inoculation, which was dramatically lower than that of the control (24.80 mg/g). Protein content in pupae infected by B. bassiana dropped from 36.57 mg/g at 12 h to 10.42 mg/g at 72 h after inoculation, which was significantly lower than that of the control (38.61 mg/g)(P＜0.05). These results showed that the protein content in different developmental stages of RIFA treated with B. bassiana declined dramatically.