Transesterification of phosphatidylcholine with eicosapentaenoic acid ethyl ester using phospholipase A2 in organic solvent |
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| Authors: | Chang Won Park Seok Joon Kwon Jeong Jun Han Joon Shick Rhee |
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| Institution: | (1) Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Kusong-dong, Yusong-gu, Taejon, 305-701, Korea;(2) Korea Research Institute of Bioscience & Biotechnology, 52 Oun-dong, Yusong-gu, Taejon, 305-333, Korea;(3) Doosan Venture BG Biotech., 69-1, Sungbok-ri, SuJi-eup, YongIn-si, Kyung gi-do, 449-840, Korea |
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| Abstract: | Phospholipase A2-catalysed transesterification of phosphatidylcholine (PC, 99%) with eicosapentaenoic acid ethyl ester (EPAEE, 95%) was carried out in organic solvent. The maximum yield was 14.3% (w/w). The optimum reaction condition was 50 °C, 48 h, initial water activity 0.25 and molar ratio of PC to EPAEE 1:10 in 5 ml toluene. |
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| Keywords: | enzymatic modification phospholipase A2 PUFA transesterification water activity |
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