Yeast invertase: Subcellular distribution and possible relationship between the isoenzymes |
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Authors: | Luis Rodríguez Teresa Ruiz Julio R. Villanueva Rafael Sentandreu |
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Affiliation: | (1) Departmento de Microbiología, Instituto de Microbiologia Bioquímica, CSIC, Facultades de Farmacia y Ciencias, Universidad de Salamanca, Salamanca, Spain |
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Abstract: | The intracellular invertase ofSaccharomyces cerevisiae is mainly found in a soluble form (91–95%), while only minor amounts are found bound to the internal (4–8%) and plasma membranes (less than 1%). In the processes of derepression or repression, inhibition of RNA or protein synthesis, or in the presence of 2-deoxy-d-glucose, the levels of the membrane-bound and external activities are modified in a way in which their relation is clear, while the soluble enzyme does not change at all. These results, together with the fact that the membrane-bound and the external enzymes are glycoproteins, suggest a precursor-product relationship between the enzymic forms. |
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