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优化细胞表面组分与结构以提高P.Putida 5-x细胞的重金属吸附能力
引用本文:吴晓林,王磊,桂晓琳,李风亭. 优化细胞表面组分与结构以提高P.Putida 5-x细胞的重金属吸附能力[J]. 工业微生物, 2004, 34(4): 23-28
作者姓名:吴晓林  王磊  桂晓琳  李风亭
作者单位:同济大学污染控制与资源化研究国家重点实验室,上海,200092;复旦大学生命科学学院,上海,200433
摘    要:研究了生物吸附剂Pseudomonas putida 5-X细胞的最佳制备条件,以提高其对重金属的吸附能力。并用Pseudomonas putida 5-X细胞为生物吸附剂处理含铜离子城市污水。实验结果表明,在优化的条件下,Pseudomonas putida 5-x细胞对铜离子的吸附容量可达87.3mg/g,经生物吸附过程处理后,废水中Cu^2 的含量降低至微量级,而且该吸附剂可有效循环使用5次。

关 键 词:细胞培养条件  细胞生长相  细胞预处理  Cu2+吸附  Pseudomonas putida

Optimization of cell surface component and structure for enhancing adsorption capability of heavy metal by P.putida 5-x cell
WU Xiao-lin ,WANG Lei ,GUI Xiao-lin ,LI Feng-ting. Optimization of cell surface component and structure for enhancing adsorption capability of heavy metal by P.putida 5-x cell[J]. Industrial Microbiology, 2004, 34(4): 23-28
Authors:WU Xiao-lin   WANG Lei   GUI Xiao-lin   LI Feng-ting
Affiliation:WU Xiao-lin 1,WANG Lei 1*,GUI Xiao-lin 2,LI Feng-ting 1
Abstract:Cells of P. putida 5-x as biosorbent were developed to remove Cu 2+ from municipal wastewater. In order to enhance the adsorption capacity of P. putida 5-x cell, the preparation conditions for P. putida 5-x cell as biosorbent was studied. Under the optimal conditions, the Cu 2+ adsorption capacity of the P. putida 5-x cell reached 87.3 mg/g. The experimental results indicated that after treatment by a semi-continuous biosorption system using optimized P. putida 5-x cell as biosorbent, the Cu 2+ concentration in wastewater was reduced to trace level.
Keywords:culture condition  cell growth phase  cell pretreatment  Cu 2+ adsorption  Pseudomonas putida
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