The importance of phenol oxidase activity in lignin degradation by the white-rot fungus Sporotrichum pulverulentum |
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Authors: | Paul Ander Karl-Erik Eriksson |
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Affiliation: | (1) Chemistry Department, Swedish Forest Products Research Laboratory, Box 5604, S-114 86 Stockholm, Sweden |
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Abstract: | The lignin degradation abilities of wildtype, a phenol oxidase-less mutant and a phenol oxidase-positive revertant of Sporotrichum pulverulentum were compared to determine if phenol oxidase activity is necessary for lignin degradation by white-rot fungi. The phenol oxidase-less mutant was unable to degrade kraft lignin or wood. The phenol oxidase-positive revertant, however, regained the ability of the wildtype to degrade kraft lignin and all of the major components of wood. It was found that kraft lignin and lignin-related phenols decreased cellulase and xylanase production by the phenol oxidase-less mutant. Addition of highly purified laccase increased the production of endo-1,4--glucanase in the phenol oxidase-less mutant in the presence of vanillic acid and kraft lignin. After addition of laccase to kraft lignin agar plates, the phenol oxidase-less mutant could degrade kraft lignin.It is proposed that phenol oxidase function in regulating the production of both lignin-and polysaccharide-degrading enzymes by oxidation of lignin and lignin-related phenols when S. pulverulentum is growing on wood.Abbreviation WT wildtype Sporotrichum pulverulentumResearch supported by a grant from Stiftelsen Nils and Dorthi Troëdssons forskningsfond |
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Keywords: | Phenol oxidases Lignin degradation White-rot fungus Sporotrichum pulverulentum Phenol oxidase-less mutant Phenol oxidase-positive revertant Cellulase Xylanase Celloblose quinone oxidoreductase Wood degradation |
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