| 球形红杆菌积累聚β-羟基链烷酸(PHA)的研究 |
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| 引用本文： | 钱新民,王宇新,常钟. 球形红杆菌积累聚β-羟基链烷酸(PHA)的研究[J]. 微生物学报, 1995, 0(6) |
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| 作者姓名： | 钱新民 王宇新 常钟 |
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| 作者单位： | 山东大学微生物研究所，山东大学微生物研究所，山东大学微生物研究所 济南 250100，济南 250100，济南 250100 |
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| 摘 要： | 通过对球形红杆菌(Rhodobacter sphaeroides)生长和积累聚卢-羟基链烷酸(PHA)条件的研究,确定采用两段培养法提高PHA的产量。第一阶段提供适合菌体生长的条件:以葡萄糖作碳源,尿素为氮源,光照微好氧培养。第二阶段则提供使菌体积累PHA的条件:补加乙酸钠厌氧光照培养。经两段培养后菌体PHA含量可占细胞干重的45％,PHA产量每升发酵液可达1.7g。
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| 关 键 词： | 球形红杆菌 聚β-羟基链烷酸 两阶段培养 |
IMPROVEMENT OF CULTURE CONDITIONS FOR ACCU-MULATION OF POLY (3-HYDROXYALKANOATES) BY RHODOBACTER SPHAEROIDES |
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| Qian Xinmin Wang Yuxin Chang Zhong. IMPROVEMENT OF CULTURE CONDITIONS FOR ACCU-MULATION OF POLY (3-HYDROXYALKANOATES) BY RHODOBACTER SPHAEROIDES[J]. Acta microbiologica Sinica, 1995, 0(6) |
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| Authors: | Qian Xinmin Wang Yuxin Chang Zhong |
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| Abstract: | Through investigating the culture conditions of Rhodobacter sphaeroi-des, a two-stage culture technique was developed for the accumulation of poly (3-hydroxyalkanoates) (PHA) by this microorganism. In the first stage, to provide suitable conditions for the microbial growth glucose and urea were supplied as carbon and nitrogen sources, respectively, keeping a microaerobic light culture. In the second stage, the culture was supplemented with sodium acetate to create an anaerobic light culture condition for the accumulation of PHA. By this technique, the PHA content accumulated in the microbial cells mounted up to 45% of cellular dry weight, and a high yield of 1.7g PHA/L fermenting liquor was achieved. |
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| Keywords: | Rhodobacter sphaeroides Poly (3-hydroxyalkanoates) Two-stage cu- lture technique |
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