Fine structure of isolated mesophyll protoplasts of tobacco |
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Authors: | Itaru Takebe Yoshiaki Otsuki Yohachiro Honda Takeshi Nishio Chiaki Matsui |
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Affiliation: | (1) Institute for Plant Virus Research, 959 Aobacho, 280 Chiba, Japan;(2) Plant Pathology Laboratory, Faculty of Agriculture, Nagoya University, 464 Nagoya, Japan |
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Abstract: | Summary Protoplasts of palisade cells isolated enzymatically from mature leaves of tobacco were studied with the electron microscope. A cell wall was completely absent, and the chloroplasts contained large inclusion bodies which were believed to be a crystalline form of fraction I protein. The fine structure of the protoplasts was otherwise that of healthy mesophyll cells, indicating that they are in a good physiological state. Some protoplasts were multinucleate as a result of fusion during the isolation process. |
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