Synthesis of two distinct α-glucosidase activities in Bacillus licheniformis |
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| Authors: | JOYCE MOORE F. G. PRIEST |
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| Affiliation: | Department of Brewing and Biological Sciences, Heriot-Watt University, Chambers Street, Edinburgh EH1 1HX, UK |
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| Abstract: | When grown with maltose or starch as carbon source at 37°C, Bacillus licheniformis synthesized two α-glucosidases which could be resolved by polyacrylamide gel electrophoresis. One of these enzymes preferentially hydrolysed p -nitrophenyl-α-D-glucoside ( p NPG) and the other was inactive on p NPG but hydrolysed maltose (maltase). Only the maltase could be detected in induced cells grown at 50°C. |
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