Study of mixed Langmuir-Blodgett films of immunoglobulin G/amphiphile and their application for immunosensor engineering |
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Authors: | Hou Yanxia Tlili Chaker Jaffrezic-Renault Nicole Zhang Aidong Martelet Claude Ponsonnet Laurence Errachid Abdelhamid Samitier Josep Bausells Joan |
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Institution: | IFoS, UMR CNRS, 5621 Ecole Centrale de Lyon, BP 163, 69131 Ecully Cedex, France. |
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Abstract: | Langmuir-Blodgett (LB) technique appears to be quite suitable for generating biospecific surfaces and it has potential application for fabricating biosensors. In this work, mixed Langmuir-Blodgett films of immunoglobulin G/amphiphile have been transferred onto hydrophobic silver surface previously modified by 1-octadecanethiol (ODT) SAMs. In order to obtain stable LB films, the influences of different parameters - type of amphiphile, surface pressure and pH - on the properties of mixed IgG/amphiphile monolayer, were investigated. Electrochemical properties of the engineered immunosensor have been measured by impedimetric spectroscopy. The immunosensor obtained exhibits a high sensitivity and a good specificity in a linear dynamic range from 200 to 1000 ng ml(-1). |
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