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The proliferative potential of chick embryo fibroblasts: Population doublings vs. time in culture
Affiliation:1. Center of the Spinal Surgery, Nipponbashi Hospital, Osaka-shi, Osaka, Japan;2. Department of Neurosurgery, Kyoto University, Kyoto-shi, Kyoto, Japan;3. Sakuragaoka Pathological Diagnostic Clinic, Kusatu-shi, Shiga, Japan;1. University of Dresden, Department of Neurology, Fetscherstrasse 74, 01307 Dresden, Germany;2. GWD Consult, Contract Research, Mozartstrasse 2–4, 63165 Mühlheim am Main, Germany;3. TEVA Pharma GmbH, Charlottenstraße 59, 10117 Berlin, Germany;1. Animal Science Research Department, Chaharmahal and Bakhtiari Agricultural and Natural Resources Research and Education Center, AREEO, Shahrekord, Iran;2. Department of Animal Sciences, University of Florida, Gainesville, FL, United States;3. Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Iran;1. Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan;2. Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan;3. Division of Biochemical Engineering, Radioisotope Research Center, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan;1. Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, DK-5230 Odense M, Denmark;2. Orphazyme ApS, Ole Maales Vej 3, 2200 Copenhagen N, Denmark;3. Department of Molecular Biology and Genetics, University of Aarhus, DK-8000 Aarhus C, Denmark
Abstract:Chick embryo fibroblasts were maintained in a non-proliferating state for up to 150 days in medium containing reduced concentrations of fetal bovine serum. Under these conditions the total time in culture was increased from 50 to 200 days while the total proliferative potential decreased only slightly. From these experiments we conclude that the ability of chick embryo fibroblasts to proliferate in vitro is much more dependent on the number of previous in vitro doublings than on the duration of the time in culture.
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