Metabolism and chemical activation ofPhycomyces blakesleeanus spores |
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| Affiliation: | 1. TiFN, Agro Business Park 82, 6708 PW Wageningen, the Netherlands;2. Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands;3. Utrecht University, Department of Biology, Microbiology, Padualaan 8, 3584 CH Utrecht, the Netherlands;4. Leiden University, Department Molecular Microbiology and Biotechnology, Institute of Biology, Sylviusweg 72, 2333 BE Leiden, the Netherlands;5. Wageningen University, Food Microbiology, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands;1. Corbion, Gorinchem, the Netherlands;2. Westerdijk Fungal Biodiversity Institute, Utrecht, the Netherlands;3. Electronmicroscopy Center, Wageningen University and Research, Wageningen, the Netherlands;4. Laboratory of Genetics, Wageningen University and Research, Wageningen, the Netherlands |
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| Abstract: | Respiratory and other data indicated that acetate was quickly metabolized byPhycomyces spores. Azide prevented metabolism of acetate although it did not inhibit oxygen uptake by dormant spores. Azide also inhibited activation of dormant spores by acetate, suggesting that acetate metabolism was necessary for spore activation. Pyruvate uptake by the spores was very limited in culture medium but could be increased substantially by lowering the pH to 3. However, pyruvate failed to activate dormant spores event at low pH values. Therefore, the lack of pyruvate metabolism could be involved in maintaining spore dormancy. Ammonium salts at pH 9 largely activated the spores. Under such conditions no increase in trehalase activity was found in the spores. Incubations with acetate in the presence of azide yielded a stimulation of trehalase activity without triggering spore activation. Therefore, spore activation and stimulation of trehalase activity seem to be two independent phenomena inPhycomyces spores. |
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