Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb |
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| Authors: | Chang Seok Oh Min Seo Jong Ha Hong Jong-Yil Chai Seung Whan Oh Jun Bum Park Dong Hoon Shin |
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| Institution: | 1.Bioanthropology and Paleopathology Lab, Institute of Forensic Science, Seoul National University College of Medicine, Seoul 110-799, Korea;2.Department of Parasitology and Research Center for Mummy, Dankook University, Cheonan 330-715, Korea;3.Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea;4.Hangang Institute of Cultural Heritage, Seoul 143-904, Korea;5.Sangmyung University, Seoul 110-743, Korea |
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| Abstract: | Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands. By cloning, sequencing, and analysis of the amplified DNA, we obtained information valuable for comprehending genetic lineage of Ascaris prevalent among pre-modern Joseon peoples. |
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| Keywords: | Ascaris ancient DNA cytochrome b 18S rRNA Korean mummy |
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