Structural stability of green fluorescent proteins entrapped in polyelectrolyte nanocapsules |
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| Authors: | Silke Krol Fabio Cannone Michele Caccia Laura Sironi Paolo Bianchini Barbara Campanini Maddalena Collini Giuseppe Chirico Alberto Diaspro |
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| Institution: | 1. LAMBS, MicroScoBiO Research Center and Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy;2. CBM – Center for Molecular Biomedicine, Laboratory for Nanodiagnostics, Drug Delivery and Analytics, AREA Science Park – Basovizza, 34012 Trieste, Italy;3. Department of Physics, LABS, University of Milano‐Bicocca, Piazza della scienza 3, 20126 Milan, Italy;4. IFOM, FIRC Institute for Molecular Oncology Foundation, Via Adamello, 16, 20139 Milan, Italy;5. Department of Biochemistry and Molecular Biology, University of Parma, 43100 Parma, Italy |
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| Abstract: | Molecules of a green fluorescent protein mutant, GFPmut2, have been immobilized in nanocapsules, assemblies of charged polyelectrolyte multilayers, with the aim to study the effect of protein‐polyelectrolyte interactions on the protein stability against chemical denaturation. GFPmut2 proteins turn out to be stabilized and protected against the denaturating action of small chemical compounds. The nanocapsule protective effect on GFPmut2 is likely due to protein interactions with the negatively charged polymers, that induce an increase in the local rigidity of the protein nano‐environment. This suggestion is supported by Fluorescence Polarization measurements on GFPmut2 proteins bound to the NC layers. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
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| Keywords: | GFP polyelectrolyte FRET layer‐by‐layer fluorescence spectroscopy protein denaturation |
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