Occurrence of Cello-Oligosaccharides in the Apoplast of
Auxin-Treated Pea Stems |
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| Authors: | Rumi Tominaga Masahiro Samejima Fukumi Sakai Takahisa Hayashi |
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| Affiliation: | Wood Research Institute, Kyoto University, Gokasho Uji, Kyoto 611, Japan (R.T., F.S., T.H.);Department of Biomaterial Sciences, School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113, Japan (M.S.) |
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| Abstract: | Treatment of pea (Pisum sativum L.) hypocotyl segments with indole-3-butyric acid, which promotes segment elongation, increased the solubilization of both xyloglucan and cello-oligosaccharides in the apoplast of auxin-treated pea stems. The cello-oligosaccharides were isolated from the apoplastic solution with a charcoal/Celite column and were identified as cellobiose, cellotriose, and cellotetraose after subsequent thin-layer chromatography and paper electrophoresis. Cello-oligosaccharides in the apoplastic fraction were monitored using cellobiose dehydrogenase. Both xyloglucan and cello-oligosaccharides appeared to be formed concurrently within 30 min after treatment with the auxin, and the cello-oligosaccharides increased with stem elongation even after 2 h. The total activity of cellulase did not increase for up to 4 h. |
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