Analysis of serum microRNAs (miR-26a-2*, miR-191, miR-337-3p and miR-378) as potential biomarkers in renal cell carcinoma |
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| Authors: | Hauser Stefan Wulfken Lena M Holdenrieder Stefan Moritz Rudolf Ohlmann Carsten-Henning Jung Volker Becker Frank Herrmann Edwin Walgenbach-Brünagel Gisela von Ruecker Alexander Müller Stefan C Ellinger Jörg |
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| Institution: | 1. Center Laboratory, Jinshan Hospital, Fudan University, Shanghai 201508, China;2. Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China;1. Department of Internal Medicine & Institute for Cancer Research, Dongsan Hospital, Keimyung University, Daegu, Republic of Korea;2. Department of Microbiology, Pusan National University, Pusan, Republic of Korea;3. Department of Radiation Oncology, College of Medicine, Yonsei University, Seoul, Republic of Korea;4. Department of Pathology, Dongsan Hospital, Keimyung University, Daegu, Republic of Korea;5. Department of Surgery, Dongsan Hospital, Keimyung University, Daegu, Republic of Korea;6. Department of Biological Sciences, Pusan National University, Pusan, Republic of Korea |
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| Abstract: | IntroductionEmerging evidence suggest that microRNAs could serve as non-invasive biomarker for cancer patients. Our study was designed to analyze circulating serum microRNAs in patients with renal cell carcinoma (RCC).Materials and methodsSerum RNA was isolated from patients with clear cell RCC (ccRCC) and non-malignant disease; an artificial microRNA (cel-miR-39) was spiked-in prior the isolation procedure to control isolation efficiency. The levels of miR-26a-2*, miR-191, miR-337-3p and miR-378 in serum were determined using quantitative real-time PCR; the microRNA levels were normalized to cel-miR-39.ResultsFirst, miR-26a-2*, miR-191, miR-337-3p and miR-378 were quantified in serum of each 25 patients with ccRCC and non-malignant disease. The level of miR-378 was significantly increased in ccRCC patients, and thus chosen for validation. The analysis of miR-378 in the validation cohort with 117 RCC patients and 123 control subjects did not confirm a different level of miR-378. Also, miR-378 was not correlated to pT-stage, lymph node/distant metastasis, vascular invasion and Fuhrman grade.ConclusionsThe analysis of circulating serum levels of miR-26a-2*, miR-191, miR-337-3p and miR-378 is unlikely to provide helpful diagnostic/prognostic information in RCC patients. |
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