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| 粪便中大肠埃希菌的分离鉴定 | |
| 引用本文： | 杨滴,王耀兵,李冬梅,宫强,苏洁. 粪便中大肠埃希菌的分离鉴定[J]. 微生物学杂志, 2007, 27(3): 1-5 |
| 作者姓名： | 杨滴 王耀兵 李冬梅 宫强 苏洁 |
| 作者单位： | 1. 国家海洋环境监测中心,辽宁,大连,116023;大连水产学院,生命科学与技术学院,辽宁,大连,116023 2. 国家海洋环境监测中心,辽宁,大连,116023 3. 大连海事大学,环境科学与工程学院,辽宁,大连,116024 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 2004年4月采集大连金州湾沿岸畜禽养殖场猪、牛、鸡和某中学人粪便样品。经改良的生化鉴定方法鉴定,得大肠埃希菌(Escherichia coli)猪源75株、牛源78株、鸡源69株、人源68株,占粪大肠菌比率分别为85.23%、92.86%、80.23%、80.00%。为验证改良的生化鉴定方法准确性,从中随机选取8株E.coli,扩增16S rRNA序列并与Genebank中E.coli16S序列比对,确定这8株细菌与E.coli同源相似率达99%以上,进而验证改良的生化鉴定方法的可行性。 |
| 关 键 词： | 粪便 大肠埃希氏杆菌 分离鉴定 |
| 文章编号： | 1005-7021(2007)03-0001-05 |
| 修稿时间： | 2006-12-19 |
Isolation and Identification of E. coli from Feces |
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| YANG Di,WANG Yao-bing,LI Dong-mei,GONG Qiang,SU Jie. Isolation and Identification of E. coli from Feces[J]. Journal of Microbiology, 2007, 27(3): 1-5 | |
| Authors: | YANG Di WANG Yao-bing LI Dong-mei GONG Qiang SU Jie |
| Abstract: | Feces samples of swine,cattle,fowl from farms,and human from a middle school were collected from coastal area of Jinzhou Bay,Dalian City,April 2004.Through improved biochemical identification method 88,86,84,and 85 fecal coliform were isolated from the above-mentioned relevant sources respectively.75 E.coli strains were from swine,78 from cattle,69 from chicken,and 68 from human respectively.The rates of E.coli over fecal coliform were 85.23%,92.86%,80.23%, and 80.00% respectively.In order to test the veracity of the results,eight E. coli strains randomly from the four sources were amplified 16S rRNA sequence and compared with 16S sequence of E.coli from Genebank.The resembling rates from the same source can exceed 99%,therefore,the improved biochemical identification method is feasible. |
| Keywords: | 16S rDNA |
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