Use of magnetic beads for Gram staining of bacteria in aqueous suspension. |
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| Authors: | S P Yazdankhah H S?rum H J Larsen G Gogstad |
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| Institution: | Department of Pharmacology, Microbiology and Food Hygiene, Norwegian School of Veterinary Science, P.O. Box 8146 Dep, N-0033, Oslo, Norway. siamak.p.yazdankhah@veths.no |
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| Abstract: | A Gram staining technique was developed using monodisperse magnetic beads in concentrating bacteria in suspension for downstream application. The technique does not require heat fixation of organisms, electrical power, or a microscope. Gram-negative and Gram-positive bacteria were identified macroscopically based on the colour of the suspension. The bacteria concentrated on magnetic beads may also be identified microscopically. |
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