Hydrolyzed fish collagen induced chondrogenic differentiation of equine adipose tissue-derived stromal cells |
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Authors: | O Raabe C Reich S Wenisch A Hild M Burg-Roderfeld H-C Siebert S Arnhold |
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Institution: | (1) Institute of Veterinary Anatomy, Histology, and Embryology, Justus-Liebig University of Giessen, Frankfurterstrasse 98, 35392 Giessen, Germany;(2) Institute of Biochemistry and Endocrinology, Faculty of Veterinary Medicine, Justus-Liebig University of Giessen, Frankfurterstrasse 100, 35392 Giessen, Germany |
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Abstract: | Adipose-derived stromal cells (ADSCs) are multipotent cells which, in the presence of appropriate stimuli, can differentiate
into various lineages such as the osteogenic, adipogenic and chondrogenic. In this study, we investigated the effect of transforming
growth factor beta 1 (TGF-β1) in comparison to hydrolyzed fish collagen in terms of the chondrogenic differentiation potential
of ADSCs. ADSCs were isolated from subcutaneous fat of horses by liposuction. Chondrogenesis was investigated using a pellet
culture system. The differentiation medium was either supplemented with TGF-β1 (5 ng/ml) or fish collagen (0.5 mg/ml) for
a 3 week period. After the 3 weeks in vitro differentiation, RT-PCR and histological staining for proteoglycan synthesis and
type II collagen were performed to evaluate the degree of chondrogenic differentiation and the formation of cartilaginous
extracellular matrix (ECM). The differentiation of ADSCs induced by TGF-β1 showed a high expression of glycosaminoglycan (GAG).
Histological analysis of cultures stimulated by hydrolyzed fish collagen demonstrated an even higher GAG expression than cultures
stimulated under standard conditions by TGF-β1. The expression of cartilage-specific type II collagen and Sox9 was about the
same in both stimulated cultures. In this study, chondrogenesis was as effectively induced by hydrolyzed fish collagen as
it was successfully induced by TGF-β1. These findings demonstrated that hydrolyzed fish collagen alone has the potential to
induce and maintain ADSCs-derived chondrogenesis. These results support the application of ADSCs in equine veterinary tissue
engineering, especially for cartilage repair. |
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