Osteoblast response to the elastic strain of metallic support |
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| Authors: | Lewandowska-Szumieł M Sikorski K Szummer A Lewandowski Z Marczyński W |
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| Institution: | 1. Department of Biophysics and Human Physiology, Medical University of Warsaw, Chałubińskiego 5, 02-004 Warsaw, Poland;2. Department of Transplantology and Central Tissue Bank, Centre of Biostructure Research, Medical University of Warsaw, Chałubińskiego 5, 02-004 Warsaw, Poland;3. Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, Poland;4. Institute of Social Medicine, Department of Epidemiology, Medical University of Warsaw, Warsaw, Poland;5. Department of Traumatology & Orthopaedics, Central Hospital Military School of Medicine, Poland;1. Curso de Farmácia, Av. José Acácio Moreira, 787, Bairro DEHON, 84, 700-900 Tubarão, SC, Brazil;2. Laboratório de Fisiopatologia Clínica e Experimental (LAFICEXP), Av. José Acácio Moreira, 787, Bairro DEHON, 84, 700-900 Tubarão, SC, Brazil;3. Programa de Mestrado em Ciências da Saúde, Av. José Acácio Moreira, 787, Bairro DEHON, 84, 700-900 Tubarão, SC, Brazil;4. Grupo de Pesquisa em Produtos Naturais (GRUPNAT). Universidade do Sul de Santa Catarina (UNISUL), Av. José Acácio Moreira, 787, Bairro DEHON, 84, 700-900 Tubarão, SC, Brazil;1. State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;2. Graduate School of Chinese Academy of Sciences, Beijing 100049, China;3. Hubei Collaborative Innovation Centre for Advanced Organic Chemical Materials and Ministry of Education, Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan 430062, China;1. Fraunhofer Institute for Production Technology IPT, Steinbachstr. 17, D-52074 Aachen, Germany;2. Bundesanstalt für Materialforschung und −prüfung (BAM), Unter den Eichen 87, D-12205 Berlin, Germany;3. Institute of Biology II, RWTH Aachen University, Worringerweg 3, D-52074 Aachen, Germany;4. Institute of Electronic Structure and Laser, Foundation for Research and Technology—Hellas, Heraklion GR-71110, Crete, Greece;1. Fraunhofer Institute for Machine Tools and Forming Technology (IWU), Reichenhainer Str. 88, 09126 Chemnitz, Germany;2. Institute for Machine Tools and Production Processes (IWP), Technische Universität Chemnitz, Reichenhainer Str. 70, 09126, Chemnitz, Germany;1. National Engineering Research Centre of Die & Mold CAD, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China;2. Department of Mechanical, Materials and Manufacturing Engineering, University of Nottingham, Nottingham, NG7 2RD, UK;3. Department of Mechanical Engineering, University of Sheffield, Sheffield, S1 3JD, UK |
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| Abstract: | It is known that metallic elements of joint endoprostheses undergo elastic strain due to their mechanical function. This is one of the factors which may be responsible for the loosening of endoprostheses. Since mechanisms involved in it remain unclear, it seems valuable to verify if cells responsible for bone regeneration are affected by a strain of the implant. Our experiment examines the influence of elastic strain applied to Ti6Al4V samples on osteoblasts cultured on their surface in vitro. Human bone-derived cells are observed in contact with metallic plates. Titanium alloy was chosen as a support since it is one of the most commonly used materials for stems in joint endoprostheses. Cyclic elastic deformation of 0.1% was applied to the support once daily for 7 days. Two thousand cycles were applied each time. Samples which were not subject to strain served as control. After the observation period XTT assay was performed, alkaline phosphatase activity as well as osteocalcin concentration and nitric oxide secretion were determined and compared with the results obtained in the control group. It was found that the number of viable cells in the mechanically stimulated population was significantly higher than in control, while both alkaline phosphatase activity and osteocalcin concentration were significantly lower in the experimental group. Nitric oxide secretion was found in the culture which was subject to elastic strain, but not in the control. The possible clinical implication is that elastic strain of the metallic endoprostheses may influence osteoblasts which are in contact with the implant in vivo. |
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