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Confirmation of the human cathepsin B gene (CTSB) assignment to chromosome 8 |
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| Authors: | Dunne Fong Marion Man-Ying Chan Wang-Ting Hsieh Joan C Menninger David C Ward |
| Institution: | (1) Department of Biological Sciences and Bureau of Biological Research, Rutgers, The State University of New Jersey, 08855-1059 Piscataway, NJ, USA;(2) Department of Biology, University of Alabama at Birmingham, 35294 Birmingham, AL, USA;(3) Clinical Neurogenetics Branch, Building 10, Room 3N218, National Institute of Mental Health, 20892 Bethesda, MD, USA;(4) Department of Human Genetics, Yale University School of Medicine, 06510 New Haven, CT, USA;(5) Department of Biological Sciences, Rutgers University, 08855-1059 Piscataway, NJ, USA |
| Abstract: | Summary Human cathepsin B gene (CTSB) has been mapped to two locations: 8p22 and 13q14. Here we confirm the chromosome 8 assignment by three independent methods: (1) analysis of human-hamster somatic cell hybrid DNA by polymerase chain reaction; (2) comparison of hybridization signals to cathepsin B in interphase nuclei of normal fibroblasts and fibroblasts with a chromosome 8 deletion; and (3) fluorescence in situ hybridization to metaphase spreads using cathepsin B cosmid clones. Our results indicate that human CTSB is located at 8p22-p23.1. |
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