Hematopoietic reconstitution of CD34+ cells derived from short-term cultured cord blood mononuclear cells |
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Authors: | Shi Yang Haibo Cai Huili Jin Jinli Fan Wen-song Tan |
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Institution: | (1) The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China; |
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Abstract: | Ex vivo expansion of hematopoietic stem cells (HSCs) is very important for clinical applications of cord blood (CB). With the aim
to find proper culture duration for ex vivo expansion, mononuclear cells (MNC) was applied as starting culture cells to expand HSCs and the repopulating potential of
seven-day and fourteen-day cultured CD34+ cells were compared. The average expansion of total cells and CD34+ cells cultured for 7 days were higher than those cultured for 14 days. The results of phenotypic analysis of fresh and cultured
cells showed that the percentage of CD3+ cells declined and the percentage of CD33+ cells increased during culture. The engraftment levels of fourteen-day cultured CD34+ cells were higher than those of fresh and seven-day cultured CD34+ cells. Fourteen-day cultured CD34+ cells also showed better multilineage reconstitution ability than fresh and seven-day cultured CD34+ cells. The results of the present study demonstrated that prolonged culture could preserve the hematopoietic reconstitution
ability of ex vivo cultured CB cells and improve the engraftment level in NOD/SCID mice. |
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