High-performance liquid chromatography of peptide bioregulators, their fragments and derivatives. I. Chromatographic pattern of corticotropin fragments of Zorbax ODS sorbent |
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Authors: | V D Grigor'ev V D Shats L A Brivkalne G I Chipens |
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Abstract: | The influence of acetonitrile concentration in the eluent and of the peptide hydrophobicity on the capacity factors has been studied. The equation is proposed that describes retention as a function of the eluent characteristics and the peptide composition. The hydrophobicity increments for -COOH, -NH2, and greater than CHCONH-fragments in the studied chromatographic system have been determined. The proposed model of peptide retention is useful for a prior evaluation of the eluent composition that is necessary to elute a compound at a given capacity factor. It can be also used for the qualitative interpretation of peptide chromatograms. |
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