Differential expression of iron metabolism proteins in diabetic and diabetic iron-supplemented rat liver |
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| Authors: | Silva Maísa de Brito Magalhães Cintia Lopes de Paula Oliveira Riva Silva Marcelo Eustáquio Pedrosa Maria Lucia |
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| Affiliation: | 1. Programa de Pós gradua??o em Ciências Biológicas, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil;2. Departamento de Ciências Biológicas, ICEB, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil;3. Departamento de Biodiversidade, Evolu??o e Meio Ambiente, ICEB, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil;4. Departamento de Alimentos, ENUT, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil |
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| Abstract: | Diabetes mellitus is associated with altered iron homeostasis that can potentially effect reactive oxygen species generation and contribute to diabetes-related complications. We investigated, by quantitative polymerase chain reaction, whether the expression of liver hepcidin, ferritin, and TfR-1 is altered in diabetes. Rats in the control (C) group received a standard diet; control iron (CI) group received a standard diet supplemented with iron; diabetic (D) group received an injection of streptozotocin; and diabetic iron (DI) group received streptozotocin and the diet with iron. Animals of the D group showed higher levels of serum iron, increased concentration of carbonyl protein, and a decrease in antioxidant status. Group D rats showed increased hepatic expression of Trf-1 compared to the other groups. Iron supplementation reversed this increase. Hepcidin mRNA was 81% higher in DI than in C and CI rats. The results suggest that diabetes, with or without excess iron, can cause perturbations in iron status, hepcidin and Trf-1 expression. |
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| Keywords: | Carbonyl iron Streptozotocin Hepcidin Ferritin Transferrin receptor |
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