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Development and characterization of microsatellite markers for Psychotria tenuinervis (Rubiaceae), a shrub species from the Atlantic forest, and primers transferability from Coffea |
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| Authors: | Paula Feliciano de Lima Flávio Nunes Ramos Maria Imaculada Zucchi Milene Möller Regina Helena Geribello Priolli Carlos Augusto Colombo Vera Nisaka Solferini |
| Affiliation: | 1. Centro de Pesquisa e Desenvolvimento em Recursos Genéticos Vegetais, Instituto Agron?mico de Campinas, C. P. 28, Campinas-SP, 13075-630, Brazil 2. Universidade Federal de Alfenas – UNIFAL-MG, Alfenas-MG, 37130-000, Brazil 3. Departamento de Genética, Universidade de S?o Paulo-USP, C. P. 83, Piracicaba-SP, 13400-970, Brazil 4. Departamento de Genética e Evolu??o, Instituto de Biologia, Universidade de Campinas-UNICAMP, C. P. 6010, Campinas-SP, 13083-970, Brazil |
| Abstract: | The shrub species Psychotria tenuinervis (Rubiaceae) is native to the Brazilian Atlantic forest and is largely found within natural and disturbed forest fragments. Aiming to develop studies on population genetic structure of forest fragment species, eigth microsatellite markers were developed for P. tenuinervis. Also, 15 loci already developed for Coffea (Rubiaceae) were tested for transferability to this species. We utilized 45 individuals from natural populations of three different fragments-anthropic edge, interior fragment and natural edge, within the Brazilian Atlantic forest. The average number of alleles per locus was 2.5 (two–four alleles/locus). These loci will be useful for future population genetic studies aiming to the conservation and management of this species. |
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