一种适于PCR扩增的小麦基因组DNA快速提取法

许多小麦分子生物学研究需要对大量的小麦样品进行PCR检测，因此，建立一种快速提取小麦基因组DNA的方法十分必要。根据国外报道的一种快速提取水稻和玉米基因组DNA的方法，我们对部分提取步骤进行变动后，在小麦上进行了尝试，长度为1．5kb的片段能得到稳定的扩增。该方法样品研磨在1.5ml的离心管内进行，后续操作不用酚、氯仿、CTAB、SDS和巯基乙醇，整个提取过程不需要使用通风橱，操作步骤简单，花费时间少，而且提取的小麦基因组DNA完整性好，量也较可观。一个DNA样品可供50～100次PCR反应使用，适用于小麦遗传多样性、分子标记辅助选择、转基因后代检测以及引物筛选、分子标记定位等多种研究。

A Rapid Isolation Method of Wheat DNA Suitable for PCR Analysis

A lot studies in wheat molecular biology need to do PCR testing to many accessions of wheat samples. Thus it is very necessary to establish a rapid DNA isolation in wheat. A rapid DNA isolation method built in rice and maize was tried in wheat after performing some modifications. The modified DNA extraction method needs even less time. With the DNA isolated with this method, 1.5kb fragment could be amplified stably. Besides the DNA quantity extracted with this method was quite considerable, one sample could meet 50-100 PCR reactions. So the DNA samples from this rapid isolation method can also be used to do primer selection, gene mapping or other PCR assays.