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Genetic variability in Metarhizium flavoviride revealed by telomeric fingerprinting
Authors:Peter W Inglisa  Bonifácio P Magalhãesa  MCléria Valadares-Inglisa
Institution:Microbial Molecular Genetics Laboratory (LGM), EMBRAPA Recursos Genéticos e Biotecnologia (CENARGEN), C.P. 02372, Brasília, D.F., CEP 70770-900, Brazil
Abstract:Previous studies using arbitrarily primed PCR (AP-PCR/RAPD) analysis have shown only little genetic variation among isolates of the entomopathogenic fungus Metarhizium flavoviride. In the current study, however, telomeric fingerprinting unambiguously differentiated several Brazilian strains of M. flavoviride as well as strains from Africa and Australia. Using this technique, similarity estimates of telomeric DNA among distinct strains were less than 50%, showing this locus to be highly mutable in this species.
Keywords:Entomopathogenic fungus  Biological control  Restriction fragment length polymorphism  Telomere  Polymorphism  Grasshopper
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