Genetic variability in Metarhizium flavoviride revealed by telomeric fingerprinting |
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Authors: | Peter W Inglisa Bonifácio P Magalhãesa MCléria Valadares-Inglisa |
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Institution: | Microbial Molecular Genetics Laboratory (LGM), EMBRAPA Recursos Genéticos e Biotecnologia (CENARGEN), C.P. 02372, Brasília, D.F., CEP 70770-900, Brazil |
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Abstract: | Previous studies using arbitrarily primed PCR (AP-PCR/RAPD) analysis have shown only little genetic variation among isolates of the entomopathogenic fungus Metarhizium flavoviride. In the current study, however, telomeric fingerprinting unambiguously differentiated several Brazilian strains of M. flavoviride as well as strains from Africa and Australia. Using this technique, similarity estimates of telomeric DNA among distinct strains were less than 50%, showing this locus to be highly mutable in this species. |
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Keywords: | Entomopathogenic fungus Biological control Restriction fragment length polymorphism Telomere Polymorphism Grasshopper |
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