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Preparation of Single Cell Detritus from Laminaria saccharina as a Hatchery Diet for Bivalve Mollusks
Authors:Pérez?Camacho  author-information"  >  author-information__contact u-icon-before"  >  mailto:alejandro.perez@co.es"   title="  alejandro.perez@co.es"   itemprop="  email"   data-track="  click"   data-track-action="  Email author"   data-track-label="  "  >Email author,J.M.?Salinas,C.?Fuertes,M.?Delgado
Affiliation:(1) Instituto Español de Oceanografía, Muelle de Ánimas s/n, 15001 La Coruña, Spain;(2) Instituto Español de Oceanografía, Promontorio de San Martín, 3900 Santander, Spain
Abstract:A high-yield technique is described for the elaboration of single cell detritus (SCD) from Laminaria saccharina, based on the sequential action of C1H, enzymes (endoglucanases and cellulases) and 2 bacteria showing a high degree of cellobiotic, proteolytic, and alginolytic activity (CECT 5255 and CECT 5256). Over 85% of dried particles of L. saccharina were transformed into a suspension of free cell and bacterial and detrital particles after 24 hours of bacterial activity with this technique. These particles were less than 20 mgrm in diameter, constituting a suitable diet for bivalve mollusks. After 72 hours 99% of the total particulate volume consisted of particles less than 20 mgrm in diameter. Tests of hatchery diets for the seed of clam Ruditapes decussatus revealed increases of 54% and 68% for live weight and length, respectively, when SCD from L. saccharina was used as the sole dietary component compared with a live phytoplankton diet. However, SCD from L. saccharina is not a suitable food for the larvae of R. decussatus.
Keywords:Biotransformation  single cell detritus  Laminaria saccharina  hatchery diet  seaweed  Ruditapes decussatus
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