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Partial DNA sequencing of Douglas-fir cDNAs used for RFLP mapping |
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| Authors: | K D Jermstad D L Bassoni C S Kinlaw D B Neale |
| Institution: | (1) Pacific Southwest Research Station, USDA Forest Service, Institute of Forest Genetics, 2480 Carson Road, Placerville, CA 95667, USA, US;(2) Department of Environmental Horticulture, University of California, Davis, CA 95616, and Pacific Southwest Research Station, USDA Forest Service, Institute of Forest Genetics, 2480 Carson Road, Placerville, CA 95667, USA, US |
| Abstract: | DNA sequences from 87 Douglas-fir (Pseudotsuga menziesii Mirb.] Franco) cDNA RFLP probes were determined. Sequences were submitted to the GenBank dbEST database and searched for similarity against nucleotide and protein databases using the BLASTn and BLASTx programs. Twenty-one sequences (24%) were assigned putative functions; 18 of which were from plant species. Six sequences aligned with conifer genes, including genes from Douglas-fir. Similarities among the 87 sequences were revealed by analyses with FASTA, suggesting either redundancy or isoforms of the same gene. Assignment of putative functions to anonymous cDNA mapped markers will increase the understanding of structural gene organization of the Douglas-fir genome. Received: 10 April 1998 / Accepted: 29 April 1998 |
| Keywords: | Douglas-fir cDNA RFLP probes DNA sequence Similarity search Putative function |
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