Immunodetection of pectin and arabinogalactan protein epitopes during pollen exine formation of Beta vulgaris L. |
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Authors: | Anna Majewska-Sawka Maria Isabel Rodriguez-Garcia |
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Institution: | (1) Institute of Plant Breeding and Acclimatization, Bydgoszcz;(2) Departamento de Bioquimica, Biologia Celular y Molecular de Plantas, Estación Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Granada |
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Abstract: | Summary. We present the results of ultrastructural and immunocytochemical studies of sugar beet microsporocytes during the developmental
phase that begins with the first meiotic metaphase and ends with the formation of young tetrads. The most prominent feature
noted during this period of microsporogenesis was the presence of numerous cisternae of endoplasmic reticulum which frequently
lie perpendicular to the surface of the plasma membrane and eventually fuse to it. Microscopic observations have been combined
with the detection of several carbohydrate epitopes representing pectins and arabinogalactan proteins in the primexine and
incipient exine. Pectin domains that possess both low and highly methylesterified epitopes, as well as pectin side chains
enriched in (1→4)-β-D-galactose residues, are deposited in this young microspore wall. The epitopes of arabinogalactan protein
that bind to JIM13, JIM8, and LM2 antibodies are localised within the callose wall surrounding posttelophase tetrads. The
possibility of endoplasmic-reticulum involvement in the synthesis, transport, or metabolism of several microspore wall compounds
is discussed.
Correspondence and reprints: Institute of Plant Breeding and Acclimatization, Powstańców Wielkopolskich 10, 85-090 Bydgoszcz,
Poland. |
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Keywords: | : Beta vulgaris Callose Endoplasmic reticulum Exine Pectin Proteoglycan |
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