Biliary metabolites of all-trans-retinoic acid in the rat |
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Authors: | K L Skare H F DeLuca |
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Institution: | Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin—Madison, 420 Henry Mall, Madison, Wisconsin 53706 USA |
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Abstract: | Biliary metabolites from physiological doses of all-trans-10-3H]retinoic acid were examined in normal and vitamin A-deficient rats. The bile from normal and vitamin A-deficient rats contained approximately 60% of the administered dose following a 24-h collection period. However, vitamin A-deficient rats show a 6-h delay in the excretion of radioactivity compared to normal rats. Retinoyl-beta-glucuronide excretion was particularly sensitive to the vitamin A status of the rats. In normal rats, retinoyl-beta-glucuronide reached a maximum concentration of 235 pmol/ml of bile 2 h following the dose and then rapidly declined. Vitamin A-deficient rats show a relatively constant concentration of this metabolite (100-150 pmol/ml of bile) over a 10-h collection period. Retinoic acid excretion was low in both normal and deficient rats. The concentration of retinotaurine, a recently identified biliary metabolite, was approximately equal to retinoyl-beta-glucuronide in normal rats and appeared in the bile 2 h later than the glucuronide. |
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Keywords: | Author to whom correspondence should be addressed |
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